Construction and production of lentiviral vectors

The vectorology platform (iVector) creates and produces customised viral and non-viral tools for gene transfer.

Lentiviral vectors are derived from the human immunodeficiency virus (HIV). They have the capacity to integrate their genome into a cell (max 8kb) but have been stripped of their replicative capacity. As a result, the DNA integrated into the cell will be transmitted during cell division to the daughter cells without the production of virus particles.

Production is carried out by transient transfection of helper plasmids and the plasmid containing your transgene into human cells. The viral particles will be produced by the use of cellular machinery and recovered by ultracentrifugation.
You can entrust us with the construction of your plasmid-transgene, or give us your already constructed plasmid directly.

3 sizes of production are proposed to you:

• mini production: about 50µL of viral vector;
• maxi production: about 150 to 200µL of viral vector;
• giga production: about 300 to 400µL of viral vector.

3 types of titrations are available:

• ELISA titration for the detection of the P24 capsid protein (quantification of the quantity of physical particles);
• titration by qPCR after cell infection (Quantification of the number of viral particles capable of infecting a cell);
• flow cytometry titration if a fluorochrome is present (Quantification of the number of infected cells able to express the integrated transgene).